Establishment of drug sensitivity model of Sorafenib in hepatocellular carcinoma cell lines with different metastatic potential
Objective:To investigate the drug sensitivity of Sorafenib in hepatocellular carcinoma(HCC)cell lines with different metastatic potential and in HCC xenografts generated in nude mice and to establish a drug sensitivity model of Sorafenib in HCC.Methods:The drug sensitivity of Sorafenib in HCC cell lines SMMC-7721(low-metastatic potential)and MHCC-97H(high-metastatic potential)and corresponding HCC xenografts generated in nude mice was detected by ATP anti-cancer drug sensi-tivity assay.Results:According to the sensitivity criterion,the IC50 value of Sorafenib in SMMC-7721 cell line group was sig-nificantly lower than that in MHCC-97H cell line group.Sorafenib was highly sensitive in SMMC-7721 xenograft group and moderately sensitive in MHCC-97H xenograft group.The chemotherapy sensibility index(CSI)and AUC values of Sorafenib in SMMC-7721 cell line group and the corresponding xenograft group were both better than those in MHCC-97H cell line and the corresponding xenograft groups,respectively.The growth inhibition rate of Sorafenib in SMMC-7721 cell line and corre-sponding xenograft groups was higher than that in MHCC-97H groups.Conclusion:Sorafenib exhibited varying drug sensitivity,CSI and AUC values,as well as growth inhibition rates in the SMMC-7721 cell line with low-metastatic potential and the MHCC-97H cell line with high-metastatic potential,particularly evident in the respective xenograft models established in nude mice.The drug sensitivity model of Sorafenib in HCC cell lines with different metastatic potential was successfully established.
NETL
NSTL
万方数据
