不同转移潜能肝细胞肝癌细胞系索拉菲尼药物敏感性研究模型建立
Establishment of drug sensitivity model of Sorafenib in hepatocellular carcinoma cell lines with different metastatic potential
杨一丹 1张冯晴 2黄思妍 2周家名1
作者信息
- 1. 南通大学医学院病理学系,江苏 226001;南通大学杏林学院医学部
- 2. 南通大学杏林学院医学部
- 折叠
摘要
目的:探讨索拉菲尼在不同转移潜能肝细胞肝癌(hepatocellular carcinoma,HCC)细胞系及荷HCC裸鼠移植瘤中的药物敏感性,建立HCC索拉菲尼药物敏感性研究模型.方法:通过ATP抗癌药物敏感性实验检测索拉菲尼在HCC细胞株SMMC-7721(惰性转移潜能)和MHCC-97H(高转移潜能)及对应荷HCC裸鼠移植瘤中的药物敏感性.结果:对照敏感性判定标准,SMMC-7721 细胞株组索拉菲尼IC50 值显著低于MHCC-97H细胞株组.荷SMMC-7721 裸鼠移植瘤组索拉菲尼高度敏感,荷MHCC-97H裸鼠移植瘤组索拉菲尼中度敏感.SMMC-7721 细胞株及对应裸鼠移植瘤组,索拉菲尼药物化疗敏感指数(chemotherapy sensibility index,CSI)及AUC值均优于MHCC-97H细胞株及对应裸鼠移植瘤组.SMMC-7721 细胞株及对应裸鼠移植瘤组中,索拉菲尼药物生长抑制率均高于MHCC-97H组.结论:索拉菲尼在惰性转移潜能SMMC-7721 细胞株和高转移潜能MHCC-97H细胞株,尤其在对应裸鼠移植瘤内,药物敏感性、CSI及AUC值、生长抑制率有差别.不同转移潜能HCC细胞系索拉菲尼药物敏感性研究模型初步建立成功.
Abstract
Objective:To investigate the drug sensitivity of Sorafenib in hepatocellular carcinoma(HCC)cell lines with different metastatic potential and in HCC xenografts generated in nude mice and to establish a drug sensitivity model of Sorafenib in HCC.Methods:The drug sensitivity of Sorafenib in HCC cell lines SMMC-7721(low-metastatic potential)and MHCC-97H(high-metastatic potential)and corresponding HCC xenografts generated in nude mice was detected by ATP anti-cancer drug sensi-tivity assay.Results:According to the sensitivity criterion,the IC50 value of Sorafenib in SMMC-7721 cell line group was sig-nificantly lower than that in MHCC-97H cell line group.Sorafenib was highly sensitive in SMMC-7721 xenograft group and moderately sensitive in MHCC-97H xenograft group.The chemotherapy sensibility index(CSI)and AUC values of Sorafenib in SMMC-7721 cell line group and the corresponding xenograft group were both better than those in MHCC-97H cell line and the corresponding xenograft groups,respectively.The growth inhibition rate of Sorafenib in SMMC-7721 cell line and corre-sponding xenograft groups was higher than that in MHCC-97H groups.Conclusion:Sorafenib exhibited varying drug sensitivity,CSI and AUC values,as well as growth inhibition rates in the SMMC-7721 cell line with low-metastatic potential and the MHCC-97H cell line with high-metastatic potential,particularly evident in the respective xenograft models established in nude mice.The drug sensitivity model of Sorafenib in HCC cell lines with different metastatic potential was successfully established.
关键词
肝细胞肝癌/索拉菲尼/药物敏感性模型/不同转移潜能/裸鼠Key words
hepatocellular carcinoma/Sorafenib/drug sensitivity model/different metastatic potential/nude mouse引用本文复制引用
基金项目
江苏省大学生创新创业训练计划项目(202313993010Y)
南通大学杏林学院自然科学基金项目(2016K135)
出版年
2024
NETL
NSTL
万方数据