The expression and clinical significance of NEK6 in ovarian cancer
Objective:To detect the expression of never in mitosis gene A-related expressed kinase 6(NEK6)in ovarian cancer tissue,and to investigate the correlation between its expression and clinical-pathological parameters as well as prognosis.Methods:Collecting tumor and normal tissues from clinical ovarian cancer patients,the real-time quantitative PCR(RT-qPCR)and Western Blot assays were used to detect the expression of NEK6 in ovarian cancer and normal ovarian tissues,and immunohistochemistry was used to detect the expression of NEK6 in 110 ovarian cancer patients.The relationship analysis was performed between its expression and age,pathological type,tumor stage,tissue grading,and metastasis.Kaplan-Meier survival curve was used to analyze the relationship between NEK6 expression in ovarian cancer and patient survival.Results:RT-qPCR,Western Blot,and immunohistochemistry results all showed that NEK6 levels in the tissues of ovarian cancer were significant higher than those in normal ovarian tissues(all P<0.01).The expression level of NEK6 was positively correlated with the tissue grading(P=0.008)and metastasis(P=0.006)of ovarian cancer.Kaplan-Meier analysis showed that patients with high expression of NEK6 had significantly lower overall survival than those with low expression(all P<0.01).Conclusion:NEK6 was highly expressed in the tumor tissue of ovarian cancer patients and affects the metastasis of ovarian cancer,suggesting that NEK6 may be involved in the occurrence and development of the ovary and can serve as a potential prognostic indicator for ovarian cancer.
ovarian cancernever in mitosis gene A-related expressed kinase 6prognosis
NETL
NSTL
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