Preparation of hyaluronic-heparin scaffold capable of sustainably releasing BMP-2 and the study of its influence on the differentiation of dental pulp stem cells
Objective:In order to enable hyaluronic acid(HA)to load and release growth factors,a hyaluronic acid-heparin(HA-Hep)scaffold was designed as a sustained-release carrier for bone morphogenetic protein 2(BMP-2),and the effect of the scaffold on odontogenic differentiation of dental pulp stem cells(DPSCs)was studied.Methods:HA-Hep materials with different ratios were prepared using chemical methods.The connection position and efficiency of heparin and hyaluronic acid were detected by Fourier transform infrared spectroscopy and elemental analysis.The microstructure and structural characteristics of the materials were observed by scanning electron microscope.BMP-2 was loaded into HA-Hep by electrostatic adsorption method,and its release curve was measured and drawn.To evaluate the biocompatibility of HA-Hep by detecting the proliferation of DPSCs.The expression levels of odontogenic differentiation-related genes were detected by qPCR to evaluate the effect of HA-Hep loading BMP-2 on odontogenic differentiation of dental pulp stem cells.Results:Microscopically,HA-Hep showed a loose porous network structure.The release of BMP-2 was sustained for 28 days,and the release rate slowed down with the increase of heparin content.HA-Hep promoted the proliferation of DPSCs was positively correlated with the materials concentration.qPCR results showed that the expression levels of dentin sialophosphoprotein(DSPP),type Ⅰ collagen(Col-1),and dentin matrix protein-1(DMP-1)in BMP-2-loaded HA-Hep material group were significantly higher than those in the control group at 7 and 14 days(all P＜0.001).Conclusion:Heparin-modified HA can delay the release of BMP-2.HA-Hep loaded with BMP-2 can up-regulate the expression of odontoblast genes(DSPP,Col-1,and DMP-1)in DPSCs,which contributes to the long-term effect of growth factors in cell differentiation.
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维普
