The influence of α-asarone in biological behavior of esophageal cancer Eca-109 cells by up-regulating expression of cytochrome C and Caspase-3
Objective:To analyze influence of α-asarone affects biological behavior of esophageal cancer Eca-109 cells by up-regulating expression of cytochrome C(cytC)and Caspase-3.Methods:Eca-109 cells of esophageal cancer were randomly divided into four groups,low dose,medium dose and high dose groups(administered 25,50 and 100 mg/L of α-asarone,respectively),and the same amount of cells severed as the blank group without α-asarone.After 48 hours of culture,the cell proliferation of four groups were detected with plate cloning experiment and apoptosis of cells was detected with Annexin V/PI method,and cytC and Caspase-3 protein and mRNA in cells were detected by RT-qPCR and Western Blot.Results:(1)The number of cell clones was the highest in the blank group,and the low dose group was significantly higher than the high dose group.The apoptosis rate of the four groups was the lowest in the blank group,and the low dose group was significantly lower than the high dose group.(2)Four groups of cells migrated to the cell number blank group＞low dose group＞medium dose group＞high dose group.(3)The expression of cytC and Caspase-3 protein in highdose group was significantly higher than that in blank group and low dose group,and the expression in medium dose group was higher than that in blank group.(4)The expression of cytC and Caspase-3 mRNA in each group was as follows:high dose group＞medium dose group＞low dose group＞blank group(all P＜0.05).Conclusion:α-asarone inhibits proliferation and invasion of Eca-109 cells and promotes apoptosis by up-regulating expression of cytC and Caspase-3.
α-asaronecytochrome CCaspase-3esophageal cancer Eca-109 cellproliferationapoptosis
万方数据
维普
