Distribution of Arabinogalactan Proteins and Photosynthesis Characteristics in Ziziphus jujuba Mill'Lingwuchangzao'Leaf
To reveal the distribution of arabinogalactan proteins AGPs and photosynthesis characteristics and drought adaptation characteristics in different development periods leaf of Ziziphus jujuba Mill'Lingwuchangzao',leaves of four periods were used as experimental material to study the distribution of AGPs by methods of immunofluorescence localization,and the photosynthesis characteristics and the soluble suger contents index of leaf were determined.The results showed that:① the external tangential cell walls of leaf epidermis cells in different developmental stages formed quite thick cuticles,antigen fluorescence of AGPs recognized by antibody distributed more copious.The mesophyll consisted entirely of developed palisade tissue cells,the cell walls and cell interiors of the mesophyll distributed antigen fluorescence of AGPs recognized by antibody in different periods leaf,the mesophyll was the main tissue of leaf AGPs distribution and the later distribution was enhanced.The cell walls and cell interiors of xylem and phloem of vascular bundles in main veins,lateral veins and veinlets located in the mesophyll distributed mass antigen fluorescence of AGPs recognized by antibody in different development periods,there were no antigen fluorescence of AGPs recognized by antibody in the vascular bundle sheaths and the secretory ducts in parenchyma.② The Pn and Gs of leaves showed a trend of alternating high and low,and reached the highest or higher level at 90 days after blossom;the Tr reached the highest value at 60 days after blossom and then decreased gradually;and the change trend of Ci was opposite to that of Tr.The contents of glucose and fructose showed a similar trend of gradual increase,and the content of glucose was always higher than that of fructose.The contents of sucrose decreased first and then gradually increased to the highest with the development of leaves,the increase speed and increment of sucrose content were always much higher than those of glucose and fructose,indicating that sucrose was mainly accumulated in leaves.The photosynthesis characteristics and the soluble suger contents of leaf showed obvious variations laws.All the results above indicated that there were some differences in fluorescence intensity of antigen recognized by antibody in different leaf tissues,the mesophyll was the main tissue of AGPs distribution in leaf all along,and AGPs probably participated in protection,drought resistance and nutritional support.
国家科技期刊平台
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