Screening of Phosphate-solubilizing Bacteria in Rhizosphere of Desert Plants and Their Antibacterial and Growth-promoting Effects
Screening phosphate-solubilizing bacteria from the rhizosphere of desert plants and studying their growth-promoting effects can lay a foundation for the development and utilization of microbial resources in desert areas.Plate culturing and molybdenum-antimony colorimetry methods were used to select phosphate-solubilizing bacterial strains from the rhizosphere soil of desert plants,and their growth-promotion and antibacterial activities were determined,followed by a pot inoculation test to analyze the growth-promoting effects of representative strains on Caragana sp.seedlings.The results showed that six strains capable of dissolving inorganic phosphate were screened,with phosphate-solubilizing capacities ranging from 52.82 to 63.93 mg/L,among which strain IP6 had the highest phosphate solubility.Four strains capable of dissolving organic phosphate were screened,with solubilization capacities ranging from 2.33 to 2.80 mg/L.Most phosphate-solubilizing bacteria also possessed the abilities to fix nitrogen,produce indole-3-acetic acid(IAA),and secrete siderophores.All strains could produce obvious inhibitory effects on the pathogens of Exserohilum turcica and Fusarium solani.Compared with the control,inoculation with Serratia sp.IP6 significantly increased the plant height,root length and fresh weight of Caragana sp.seedlings by 12.39%,9.85%and 30.68%,respectively;and inoculation with Pseudomonas sp.OP2 significantly increased the plant height,root length and number of leaves by 13.29%,8.62%and 19.56%,respectively,and the fresh weight and dry weight by 46.32%and 37.98%,respectively.The growth-promoting effects of both strains were significant,indicating their potential to be further developed as microbial fertilizers.
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