Effects of Hypersensitive Protein and Chitosan Oligosaccharide on the Growth,Photosynthesis,and Antioxidant Capacity of Tomato Seedlings under Brackish Water Irrigation
This study aims to explore methods for mitigating salt damage during seedling growth in brackish water and to rationally utilize hypersensitive protein(HP)and chitosan oligosaccharide(COS).The tomato variety Yulinda was used as the test material,employing three treatment stages:seed soaking,leaf spraying,and irrigation in a greenhouse seedling tray.Eight experimental treatments were established:CK1:full-stage freshwater;CK2:full-stage brackish water(EC=2.5 g/L);A1:freshwater+500 times HP+brackish water;A2:freshwater+800 times HP+brackish water;B1:200 times HP+800 times HP+brackish water;B2:400 times HP+500 times HP+brackish water;C1:200 times HP+800 times COS+brackish water;C2:400 times HP+500 times COS+brackish water.The objective was to determine the optimal concentrations and application methods for HP and COS.Results demonstrated that under brackish water irrigation,soaking seeds in freshwater or HP prior to sowing,and spraying COS or HP during the seedling stage significantly improved the growth and development of tomato seedlings.Compared to the brackish water control(CK2),the root activity in A2 increased significantly by 277.15%.The net photosynthetic rate in B2 increased significantly by 34.96%,while the contents of malondialdehyde(MDA)and proline significantly decreased.Stomatal conductance in C2 increased significantly by 8.33%,and peroxidase and catalase activities increased significantly by 221.13%and 14.64%,respectively.Principal component analysis indicated that soaking seeds in 400 times HP before sowing and spraying 500 times COS at the seedling stage provided the optimal method for alleviating salt stress in tomato seedlings.This approach is recommended for cultivating robust seedlings under brackish water conditions.
brackish watertomato seedlinghypersensitive proteinchitosan oligosaccharidegrowth and developmentphotosynthesisantioxidant capacity
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