Toxicity of Copper Oxide Nanoparticles to the Seed Germination of Chinese Cabbage
Eco-toxicity of nanomaterials was subject to more and more attention recently. The toxicity of copper oxide nanoparticles( 22-75 run, the average 43 run) to seed germination of Chinese cabbage was investigated, and the counterpart investigation of copper oxide micron particle and copper ions were compared. The results showed that the effect of copper oxide nanoparticles within the concentrations of 0.2, 0.5, 1, 2,4 mg·L-1 and 8 mg·L-1 on seed germination rate of Chinese cabbage showed no significant difference compared with control(P>0.05), but the inhibitation of root elongation and shoot elongation was observed in lower concentrations. With the increasing concentrations of copper oxide nanoparticles, inhibition rate increased significantly for root elongation but slowly for shoot elongation, with half-inhibitory concentration (IC50) of 11.4 mg·L-1 and 1 309.4 mg·L-1, respectively. The toxicity of copper oxide nanoparticles was stronger than that of copper oxide micron particle. However the copper ion(Cu2+, 0.22 mg· L-1) dissolved from 8 mg · L-1 of copper oxide nanoparticles contributed to the inhibitation only 4.6% for root elongation and almost zero for shoot elongation.