首页|纳米氧化铜对白菜种子发芽的毒害作用研究

纳米氧化铜对白菜种子发芽的毒害作用研究

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纳米材料的生态毒性问题越来越受到人们的广泛关注.本文探讨了纳米氧化铜(22~75 nm,平均43 nm)对白菜种子发芽的影响,并以微米氧化铜和铜离子(Cu2+)进行对比分析.结果表明,各浓度处理(0.2、0.5、1、2、4、8 mg·L-1)纳米氧化铜对白菜种子发芽率与对照相比均无显著差异(P>0.05),但在较低浓度下(0.4 mg· L-1)对根伸长和芽伸长即表现出抑制作用.随着纳米氧化铜浓度增加,对根伸长抑制率显著提高,对芽伸长抑制率则缓慢提高,其半抑制浓度(IC50)分别为11.4 mg·L-1和1309.4 mg·L-1.100 mg·L-1时,微米氧化铜对白菜根、芽伸长的抑制率都小于20%,其毒性小于纳米氧化铜;但纳米氧化铜的毒性并非其溶解出的铜离子所致.
Toxicity of Copper Oxide Nanoparticles to the Seed Germination of Chinese Cabbage
Eco-toxicity of nanomaterials was subject to more and more attention recently. The toxicity of copper oxide nanoparticles( 22-75 run, the average 43 run) to seed germination of Chinese cabbage was investigated, and the counterpart investigation of copper oxide micron particle and copper ions were compared. The results showed that the effect of copper oxide nanoparticles within the concentrations of 0.2, 0.5, 1, 2,4 mg·L-1 and 8 mg·L-1 on seed germination rate of Chinese cabbage showed no significant difference compared with control(P>0.05), but the inhibitation of root elongation and shoot elongation was observed in lower concentrations. With the increasing concentrations of copper oxide nanoparticles, inhibition rate increased significantly for root elongation but slowly for shoot elongation, with half-inhibitory concentration (IC50) of 11.4 mg·L-1 and 1 309.4 mg·L-1, respectively. The toxicity of copper oxide nanoparticles was stronger than that of copper oxide micron particle. However the copper ion(Cu2+, 0.22 mg· L-1) dissolved from 8 mg · L-1 of copper oxide nanoparticles contributed to the inhibitation only 4.6% for root elongation and almost zero for shoot elongation.

nanoparticlescopper oxideseed germinationtoxicityChinese cabbage

向垒、莫测辉、卢锡洪、吴小莲、李彦文、黄献培、屈相龙、郝艳、黄韵怡

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暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州510632

中山大学化学与化学工程学院,广东高校环境与能源化学重点实验室,广州510275

纳米颗粒 氧化铜 种子发芽 毒性 白菜

国家自然科学基金广东省科技计划项目广东省高校高层次人才项目广州市科技计划暨南大学第七批本科生科技创新工程项目

410712112010B02031100610A82070466CX11021

2011

农业环境科学学报
农业部环境保护科研监测所 中国农业生态环境保护协会

农业环境科学学报

CSTPCDCSCD北大核心
影响因子:1.52
ISSN:1672-2043
年,卷(期):2011.30(9)
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