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外源抗坏血酸对番茄自毒作用的缓解效应

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为探究外源抗坏血酸（AsA）提高自毒作用下番茄抗逆能力的内在机制，以优良番茄品种“粉冠一号”为材料，用连作3 a番茄的基质浸提液处理种子及幼苗，研究了不同浓度（0、0.25、0.5、1 mmol·L-1）AsA对自毒作用下番茄种子发芽率、发芽势、发芽指数和幼苗主根、上胚轴长、抗氧化酶（SOD、POD、APX）活性、抗氧化剂（AsA、GSH）含量、MDA含量和电解质渗漏率及根系超微结构的影响。结果表明：单一基质浸提液处理可抑制番茄种子的萌发和幼苗生长，降低SOD、POD和APX酶活性及GSH和AsA含量， MDA含量和电解质渗漏率升高；当用含有不同浓度AsA的基质浸提液处理后，与单一基质浸提液处理相比，番茄种子的发芽率、发芽势和发芽指数均增大，幼苗主根和上胚轴的生长加快，植株的抗氧化酶活性和抗氧化剂含量升高，MDA含量和电解质渗漏率均降低，对幼苗根系结构的不良影响减少。外源AsA能维持番茄幼苗正常的生理功能，有效缓解连茬基质浸提液对其造成的自毒胁迫。所有处理各指标的综合隶属函数值分析表明，1 mmol·L-1的AsA缓解效果最好，0.5 mmol·L-1次之。
Mitigation of Autotoxicity Stress of Tomato by Ascorbic Acid
Ascorbic acid widely present in many plant tissues is a high abundant and small molecular antioxidant. It plays an important role in protecting plant cells from oxidative damages and stimulating cell division and elongation. This experiment was carried out to explore the effects of exogenous ascorbic acid on resistance of tomato to autotoxicity. Tomato seeds were treated with substrate extracts of 3 year continu-ous tomato cropped soil, and the effects of exogenous ascorbic acid at different concentrations(0, 0.25, 0.5, 1 mmol·L-1)on the seed germi-nation and plant growth of tomato and the ultrastructure of roots were then investigated. It was found that substrate extracts suppressed seed germination, seedling growth, decreased the activities of superoxide dismutase, peroxidase, and ascorbate peroxidase, and reduced the con-tent of glutathione and ascorbic acid, but increased malondialdehyde content and relative electrolyte leakage. Compared with substrate ex-tracts, treatments with ascorbic acid at 0.25, 0.5 mmol·L-1 and 1 mmol·L-1 increased the seed germination rate, germination energy and ger-mination index, enhanced seedling root and epicotyl, elevated the protective enzyme activity and antioxidant content, decreased malondi-aldehyde content and relative conductivity. In addition, exogenous ascorbic acid could alleviate harmful effects on root structure. There re-sults indicate that supplying exogenous ascorbic acid could alleviate tomato autotoxicity caused by continuous cropping, with 1 mmol·L-1 having the best effect.

Lycopersicon esculentumsubstrate extractsascorbic acidautotoxicitygerminationultra-microstructure

马彦霞、张玉鑫、胡琳莉、吕剑、郁继华、王晓巍

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甘肃省农业科学院蔬菜研究所，兰州 730070

甘肃农业大学园艺学院，兰州 730070

番茄 基质浸提液 抗坏血酸(AsA) 自毒作用 种子萌发 超微结构

农业部西北地区蔬菜科学观测实验站项目甘肃省农业科学院院列创新专项农业部农业产业技术体系建设资金项目

2015-A2621-620321- G1203-0662014GAAS02CARS-25-C-07

2015

10.11654/jaes.2015.07.004

农业环境科学学报
农业部环境保护科研监测所 中国农业生态环境保护协会

农业环境科学学报

CSTPCDCSCD北大核心
影响因子：1.52
ISSN：1672-2043
年,卷(期)：2015.(7)
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