Uptake kinetic characteristics and subcellular distribution of Pb2+and Cd2+in Neyraudia reynaudiana
Neyraudia reynaudiana is a soil-conservating plant and can grow well in heavy metal polluted soils. Here a solution culture ex-periment was conducted to examine uptake and subcellular distrubution of Pb and Cd in the plant under different metal levels and stress times. Results showed that the uptake of Pb and Cd by N. reynaudiana increased with heavy metal concentrations and stress time. Fre-undlich and Michaelis-Menten equations fit the data very well, R2>0.95. It was also revealed that heavy metals were mainly distributed in the roots. Neyraudia reynaudiana had greater absorption ability for Pb(the highest content reached to 4 687.87 mg·kg-1), but higher absorp-tion rate for Cd. For subcellular distribution, Pb and Cd were mainly distributed in the cell walls and soluble constituents. Under different concentrations and stress times of Pb and Cd, the Pb/Cd content in cell wall and soluble constituent subcellular components accounted for over 60% of the total. Therefore, N. reynaudiana has stronger absorption ability of Pb, and it could change the subcellular distribution of heavy metals to reduce the toxicity of heavy metals for maintaining its steady state. This study provides theoretical support for screening phytoremediation materials.
Neyraudia reynaudianaPbCdabsorption kineticssubcellular distribution