Residues and dietary risk assessment of fluopyram and pyraclostrobin in grape
To determine the residue risks of fluopyram and pyraclostrobin on grapes,the supervised residue trials were conducted in 10 regions within 1 year.A highly efficient and simple method was developed for the determination of fluopyram and pyraclostrobin in grapes using high-performance liquid chromatography(HPLC).The results demonstrated that a good linear relationship between the peak area and the concentration of fluopyram and pyraclostrobin was observed in the range of 0.1-20.0 mg·L-1 with a correlation coefficient greater than 0.99.The average recoveries of fluopyram and pyraclostrobin in grapes at three spiked levels of 0.05,0.50,and 2.00 mg·kg-1 were 88.1%-112.3%and 90.5%-101.5%,with the relative standard deviations(RSDs)ranged from 3.1%-8.2%and 3.5%-9.1%,respectively.The limits of quantification(LOQs)of the two analytes in grape were all 0.05 mg·kg-1.The supervised trial median residues(STMR)of fluopyram and pyraclostrobin in grapes were 0.16 and 0.12 mg·kg-1,and the highest residues(HR)were 1.44 and 1.48 mg·kg-1,respectively,at 14 d after the last application.According to the dietary risk assessment,the contribution rates of fluopyram and pyraclostrobin in grapes to the long-term dietary risk of the general population were 0.142%and 0.036%,the short-term dietary intake risk quotients were 7.8%and 5.7%,and the short-term dietary intake risk quotients for children aged 1-6 years were 19.6%and 14.4%,respectively.These results show that the residues of fluopyram and pyraclostrobin in grapes would not pose significant health risks to children aged 1-6 years and the general population when fluopyram and pyraclostrobin formulation are applied at the recommended dose.
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