Oxidative damage of mebendazole to the liver of Paramisgurnus dabryanus
To explore the toxic effects of mebendazole via oxidative damage to the liver of Paramisgurnus dabryanus,P.dabryanus were exposed to mebendazole solution concentrations of 0(control),0.004,0.02 mg·L-1,and 0.1 mg·L-1.The activities of glutamic pyruvic transaminase(GPT),glutamic oxaloacetic transaminase(GOT),superoxide dismutase(SOD),glutathione peroxidase(GPx),and acetylcholinesterase(AChE);the total antioxidant capacity(T-AOC);and malondialdehyde(MDA)content in the liver tissue of P.dabryanus were measured at 24,72 h,and 144 h.The results showed that the activity of GOT decreased with the increase in mebendazole concentration at 24 h,but increased and then decreased at 72 h and 144 h,respectively.The activity of GPT in the low(0.004 mg·L-1),medium(0.02 mg·L-1),and high(0.1 mg·L-1)concentration groups was lower than that in the control group during the entire experiment.The SOD activity in all concentration groups showed a trend of inhibition,except for the medium concentration group at 72 h.The GPx activity and T-AOC level were significantly inhibited(P<0.05)during the entire exposure to mebendazole,whereas the MDA content was induced to varying degrees.Furthermore,at 24 h,the AChE activity in each concentration group was significantly lower than that in the control group(P<0.05),whereas at 72 h and 144 h,the AChE activity increased with the increase in mebendazole concentration;it was also significantly suppressed in the low concentration group(P<0.05)and significantly induced in the high concentration group(P<0.05).These results suggest that exposure to mebendazole can cause oxidative stress and damage to the liver tissue of P.dabryanus,interfere with the neurotransmitter system,and produce neurotoxic effects in fish.
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