Analysis of the deodorization function of Bacillus cabrianesii BCH and its application in pig manure composting
To mitigate environmental pollution caused by the odorous gases generated during the composting of livestock and poultry manure,this study took a strain of Bacillus cereus with a deodorizing effect obtained by the group′s pre-separation and screening in swine manure as an object to determine its removal ability of NH3 and H2S and so on.Genes related to nitrogen and sulfur metabolism after whole genome sequencing of strain BCH were mined.Pig manure was used as composting material to investigate the deodorizing ability of strain BCH in composting and the effect of strain on the composting of livestock and poultry manure.The research revealed that the strain was Bacillus cabrialesii,with an optimal growth temperature range of 30-60℃and an optimal pH range of 4.0-9.0.Strain′s ammonia nitrogen utilization rate reached up to 45% .In indoor pig manure experiments,the strain achieved a 27% removal rate of ammonia nitrogen and a 26% removal rate of hydrogen sulfide.Enzyme activity assays revealed that the strain exhibited various degradative enzyme activities,with lipase activity at 23.99 U·mL-1,protease activity at 36.44 U·mL-1,and the highest carboxymethyl cellulase activity at 36.52 U·mL-1.Third-generation whole-genome sequencing analysis identified nitrogen and sulfur metabolism-related genes,including hao and soxB.When strain BCH was applied to pig manure composting,the BCH group′s organic matter content,total nutrient content,and seed germination index were 64.35%,4.66%,and 151.64%,respectively,all higher than those of the CK control group.Additionally,the odor emission from the inoculated group was significantly lower than that of the CK blank group.Analysis of the changes in microbial community structure of compost samples showed that at the genus level,Sphingobium was the dominant bacterial group in the early stages of compost fermentation in both groups and as composting proceeded,the abundance of bacteria with nitrifying capacity(e.g.,Ureibacillus)and sulfur-oxidizing capacity(e.g.,Pseudoxanthomonas)had increased in the experimental groups.This study provides a basis and bacterial resources for improving the quality of livestock manure compost products.
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