Cytotoxicity of bamboo vinegar and bamboo tar on Allium cepa L.root tip
To assess the potential hazards of bamboo vinegar(BV)and bamboo tar(BT)to plants and to support the development of technical specifications for their safe use in environmental and agricultural applications,this study used onion as the test plant to investigate the cytotoxicity and genotoxicity of onion root tips treated with these substances at various concentrations.Cytotoxicity was evaluated by measuring cell relative viability and the mitosis index,while genotoxicity was assessed through chromosome aberration rates,micronucleus formation,and DNA damage.The findings revealed that both BV and BT had a"low-promoting and high-suppressing"effect on onion root tip growth.Specifically,onion roots did not grow in 10% BV or 0.2% BT solutions.After 72 h of treatment,root elongation in a 1% BV solution decreased by 97.49% .The semi-inhibitory concentrations(IC50)for BV and BT affecting onion root tip growth were 0.01% and 0.02%,respectively.While BV displayed weak genotoxicity,prolonged exposure to high concentrations still impacted mitosis and caused DNA damage.BT,on the other hand,demonstrated cytotoxicity at 0.1%,with a chromosome aberration rate of 9.09% and severe DNA damage.In conclusion,BV shows low cytotoxicity and genotoxicity to onion root tip cells,whereas BT results in significant cytotoxicity and moderate to severe genotoxicity.
万方数据
维普
