Determination of kasugamycin residues in fruits,vegetables,sugar crop,and cereal by ultra-performance liquid chromatography-tandem mass spectrometry
A ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)coupled with ion chromatography column method was developed for the determination of kasugamycin residues in fruits(apples,grapes,citrus fruits),vegetables(aubergines,pak choi,cucumber),sugar crop(sugar cane)and cereal(brown rice).The samples were extracted with water and aqueous acetic acid solution,purified on a HLB solid phase extraction column and separated on a Metrohm Metrosep A Supp 5 column(4.0 mm×150 mm,5 μm)with a gradient elution using water and 200 mmol·L-1 ammonium bicarbonate+0.05% aqueous ammonia solution as mobile phases,and then analysed by a positive electrospray ionisation(ESI+)source in a multiple reaction monitoring mode for tandem mass spectrometry analysis and an external standard method for quantification.The results showed that kasugamycin was well retained under the test conditions and exhibited good linearity(R2≥0.991)over a wide concentration range(0.001/0.002-0.5 mg·L-1),with limits of quantification(LOQs)of 0.005-0.010 mg·kg-1.The mean inter-day recoveries(n=15)of kasugamycin in each matrix at spiked levels of 0.01(0.005 for apple),0.05,0.1 and 1 mg·kg-1 ranged from 81.9% to 125.2% with relative standard deviations(RSDs)of 7.4%-20.5% .The present method is simple to use,sensitive and suitable for the determination of kasugamycin residues in various agricultural products such as fruits,vegetables,sugar crop and cereal.
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