Response surface methodology for the design and analysis of optimizing potassium and sodium ion concentration combinations to counteract the impact of low temperature on neural action potentials
In order to analyze the effects of different concentrations and orthogonal combinations of K+and Na+on the action potential of neural stem under low-temperature treatment,the main factors and optimal solutions were scientifically screened.Firstly,the neural stem was treated at different temperatures.Secondly,it was treated at 5 ℃ and soaked in potassium ion Ren's solution and sodium ion Ren's solution of different concentrations.Then,a quadratic regression orthogonal combination design experiment(response surface methodology)was used for analysis.The results show that low temperature significantly reduced the electrophysiological activity of nerve trunks.As the Na+concentration increases,the amplitude and conduction velocity of the action potential first in-crease and then decrease.As the K+concentration decreases,the amplitude and conduction velocity of the action potential first increase and then decrease.The orthogonal combination design experiment show that K+concentra-tion and Na+concentration have significant effects(P＜0.05),and the effect of Na+is greater than that of K+.There is a significant quadratic regression model(P＜0.05)between action potential amplitude Y1 and K+con-centration(X1),Na+concentration(X2).There is a significant quadratic regression model(P＜0.05)between action potential conduction velocity Y2 and K+concentration(X1)and Na+concentration(X2).The basic conclu-sion is that the optimal experimental conditions are:K+concentration(X1)2.60 mmol·L-1,Na+concentration(X2)222.4 mmol·L-1.The action potential(Y)1 of the neural stem is 14.40 mV,and the action potential con-duction velocity(Y)2 is 37.51 m·s-1.Orthogonal combination optimization of K+concentration and Na+concentra-tion can counteract or restore the effect of low temperature on neural stem action potential.
low temperaturedifferent concentrations of K+and Na+neural stemorthogonal experiment
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