Propofol regulates proliferation,migration and invasion of glioma cells through miR-301a/PI3K/AKT axis
Objective To investigate the role of propofol in glioblastoma(GBM)and its underlying mechanisms.Methods Cell proliferation was determined by cell counting Kit-8(CCK-8)and clone formation assay.Transwell assay for apoptosis and migration,invasion.Bioconfidence database and qRT-PCR were used to analyze the level of miR-301a.Protein immunoblotting was used to analyze PI3K/AKT pathway activity.Results Propofol inhibits GBM cell proliferation,migration and invasion and promotes apoptosis.miR-301a expression is upregulated in GEB tissues and cells.Propofol inhibited the malignant progression of GBM cells by suppressing miR-301a expression.In addition,miR-301a overexpression promoted the protein expression of p-P13K and p-AKT,whereas propofol treatment partially attenuated the promotional effect of miR-301a overexpression on the expression of p-P13K and p-AKT.Conclusion Propofol inhibits GBM progression by potentially mediating the miR-301a/PI3K/AKT axis,and this study could help guide the clinical use of propofol with a view to obtaining a better patient prognosis after surgical removal of the tumor.
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