p150Glued deficiency leads to striatal dopaminergic axonal degeneration of mice
Objective To explore the role of the Perry syndrome-associated protein p150Glued in midbrain dopaminergic neurons and striatal dopaminergic axons of mouse.Methods The number of midbrain dopaminergic neurons and the structure of striatal dopaminergic axons in cKO and Ctrl mice were observed by immunofluorescence staining.Results Compared to Ctrl mice,the numbers of ALDH1A1-positive and ALDH1A1-negative dopaminergic neurons were significant decreased in the midbrain of cK0 mice.The ALDHIA1-positive and ALDH1A 1-negative dopaminergic axons were also significantly lost in the striatum of cKO mice.We also observed that the striatal dopaminergic axons of cKO mice were markedly swollen,and there was abnormal accumulation of VAMT2-positive vesicles and Cathepsin D-positive structures in the swollen dopaminergic axons.Conclusion p150Glued is necessary for the survival of dopaminergic neurons and the integrity of dopaminergic axons structure in mice.Moreover,p150Glued deficiency can lead to transport dysfunction in the dopaminergic axons.
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