首页|球孢白僵菌诱导酶发酵液对其毒力的增效作用

球孢白僵菌诱导酶发酵液对其毒力的增效作用

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为进一步确认含蛋白酶、几丁质酶、脂肪酶和淀粉酶的诱导酶发酵液对球孢白僵菌( Beauveria bassiana)菌株毒力的影响，将高毒力菌株B5、Bxs与低毒力菌株B11、BZ分别制成1×107个/mL 孢悬液( I型)、孢悬液＋诱导酶发酵液( V∶V＝1∶1；II型)、孢悬液＋灭菌诱导酶发酵液( V∶V＝1∶1；III 型)，用浸渍法分别测定 I 型、II 型与 III 型3种液体对松墨天牛( Monochamus alternatus)幼虫的毒力。结果表明：分别含有蛋白酶、几丁质酶和脂肪酶的诱导酶发酵液Ⅱ型液体，其LT50值与Ⅲ型液体LT50间的差异均为极显著(P<0.01)，而含淀粉酶的差异不显著。分别含有4种酶的诱导酶发酵液的Ⅲ型与Ⅰ型液体相比，LT50值之间也存在极显著差异(P<0.01)。可见：含蛋白酶、几丁质酶和脂肪酶的诱导酶发酵液对球孢白僵菌毒力具有极显著的增效作用；经过酶灭活的4种诱导酶发酵液增效作用也极显著。
Synergistic effect of induced enzyme fermented solution on the insecticidal efficacy of Beauveria bassiana
In order to evaluate the toxicity effect of induced enzyme fermented solution of protease, chitinase, lipase and amylase from Beauveria bassiana, 3 types of solutions were prepared under laboratory conditions, i. e. , 1 × 107/mL spore suspension ( type I ) , spore suspension + induced enzyme fermented solution ( V∶V=1∶1, type II) and spore suspension + sterilized induced enzyme fermented solution ( V∶ V =1∶1 , type III ) , and microbial virulence against Monochamus alternatus larvae were tested by immersion method. The results showed that the LT50 values of induced protease, chitinase and lipase fermented solution( type II) were significantly different from that of type III solution ( P<0. 01 ) . For induced amylase fermented solution, the LT50 values was not significantly different&nbsp;between the two types of solution. Moreover, there were significant difference between type Ⅲ and type I fermented solution of 4 kinds of enzyme ( P < 0. 01 ) . The results indicated that induced protease, chitinase and lipase fermented solution had significant synergistic effect on the toxicity of B. bassiana, and the inactivated 4 kinds of fermented solution also showed significant synergistic effect.

Beaureria bassianaproteasechitinaselipaseamylaseMonochamus alternatustoxicityinduced enzyme fermented solutionsynergistic insecticidal efficacy

张旦妮、赵学、张媚、朱旭伟、张璞瑜、张爽、林海萍

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亚热带森林培育国家重点实验室培育基地，生物农药高效制备技术国家地方联合工程实验室，浙江农林大学，浙江 临安311300

球孢白僵菌 蛋白酶 几丁质酶 脂肪酶 淀粉酶 松墨天牛 毒力 诱导酶发酵液 增效作用

浙江省自然科学基金浙江省大学生科技创新活动计划暨新苗人才计划浙江省大学生科技创新活动计划暨新苗人才计划国家级大学生创新创业训练计划项目

LY12C140052014R4120332014R412049201310341003

2014

10.3969/j.issn.1008-7303.2014.05.08

农药学学报
中国农业大学

农药学学报

CSTPCDCSCD北大核心
影响因子：0.879
ISSN：1008-7303
年,卷(期)：2014.(5)
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