摘要
为明确引起山西省玉米纹枯病的主要丝核菌融合群对氟酰胺、噻呋酰胺和戊菌隆 3 种杀菌剂的敏感性,采用菌丝生长速率法测定了氟酰胺、噻呋酰胺和戊菌隆对 268 株丝核菌菌丝生长的EC50 值,建立敏感基线并分析了噻呋酰胺和其他杀菌剂对病菌活性的相关性.结果表明:立枯丝核菌Rizoctonia solani融合群AG-5 和玉蜀黍丝核菌R.zeae融合群WAG-Z对噻呋酰胺最为敏感,对氟酰胺敏感性次之,对戊菌隆敏感性最差;立枯丝核菌融合亚群AG-1-IA则对戊菌隆最为敏感,对噻呋酰胺次之,对氟酰胺最差.通过箱形图分析剔除异常的EC50 值后,融合群AG-5 和WAG-Z对氟酰胺、噻呋酰胺和戊菌隆的敏感性频率均呈连续的单峰曲线分布,符合正态分布.因此,将EC50 均值 0.165、0.048 和 2.500 μg/mL分别作为融合群AG-5 对氟酰胺、噻呋酰胺和戊菌隆的敏感基线;将 0.518、0.106 和 1.616 μg/mL分别作为融合群WAG-Z对氟酰胺、噻呋酰胺和戊菌隆的敏感基线.噻呋酰胺与氟酰胺、戊菌隆、己唑醇或咯菌腈对融合群AG-5 和WAG-Z的抑制活性不存在相关性.研究结果可为山西省玉米纹枯病杀菌剂施用策略的制定、病原菌抗性的监测及风险评估提供一定的参考依据.
Abstract
To clarify the sensitivity of the main anastomosis groups of Rhizoctonia spp.inciting maize sheath blight to flutolanil,thifluzamide,and pencycuron in Shanxi province,the EC50 values of 268 Rhizoctonia spp.were determined by mycelium growth rate method,and sensitivity baselines were established.Additionally,the correlation between thifluzamide and other fungicides on inhibitory activities to Rhizoctonia spp.was analyzed.The results indicated that the anastomosis grops of R.solani AG-5 and R.zeae WAG-Z were the most sensitive to thifluzamide,followed by flutolanil,and least sensitive to pencycuron,and R.solani anastomosis subgroup AG-1-IA were the most sensitive to pencycuron,followed by thifluzamide,and least sensitive to flutolanil.After removing the outliers of EC50 values using boxplot analysis,both AG-5 and WAG-Z exhibited a continuous unimodal distribution of sensitivity frequencies to the three fungicides,complying with a normal distribution.Therefore,the mean EC50 values of 0.165,0.048,and 2.500 μg/mL were established as the sensitivity baselines for AG-5 to flutolanil,thifluzamide,and pencycuron,respectively.Similarly,the mean EC50 values of 0.518,0.106,and 1.616 μg/mL were defined as the sensitivity baselines for WAG-Z to flutolanil,thifluzamide,and pencycuron,respectively.There was no observed correlation between thifluzamide and flutolanil,pencycuron,hexaconazole,or fludioxonil in terms of their inhibitory activity on AG-5 and WAG-Z.The results could serve as a valuable reference for the development of fungicide application strategies,monitoring and risk assessment of fungicide resistance for maize sheath blight in Shanxi Province,China.
基金项目
山西省基础研究计划(20210302124623)
山西省基础研究计划(20210302124375)
国家科技期刊平台
NSTL
维普
万方数据