Extraction and Enzymatic Removal of Protein from the Epimedium Brevicornum M.Polysaccharides
To study on the deproteinization by enzyme method for the Epimedium brevicornum M.polysaccharides,papain was used to remove protein from polysaccharides of Epimedium brevicornum M.Factors such as enzyme concentration,temperature,pH and time were evaluated in terms of the removing ratios of protein and polysaccharide.The optimal deproteinization conditions including 10％ papain,temperature 55 ℃,pH6.0 and 1 h.Conclusions papain is a appropriate deproteination method for the Epimedium brevicornum M.polysaccharides in which deproteinization rate was 90％ while 16 ％ of polysaccharide was remained.
NETL
NSTL
万方数据
维普
