Using the technique ofaccelerated strand exchange amplification(ASEA),a new method for the detection of novel coronavirus nucleic acid was established.Retrospective a-nalysis was performed on 1 492 samples from the laboratory department of Weifang Second People's hospital.The sensitivity,specificity,positive predictive value(PPV),negative predictive value(NPV),and agreement rate of the detection results of the SARS-CoV-2 nu-cleic acid detection kit were calculated.The nucleic acid detection kit could complete nucleic acid amplification within 30 min with a detection limit of 2 copies per reaction.Among the 1492 samples from patients with respiratory tract infections respiratory tract infections,the sensitivity of this kit was 96.7%,and the specificity was 99.9%.This kit for detecting SARS-CoV-2 within only 30 min,while the previously reported traditional qPCR method took several hours.We have proved a rapid nucleic acid detection kit for detecting SARS-CoV-2 with accelerating strand exchange amplification.
维普
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