Fluorescence Assay for APE1 Based on DNAzyme Assisted Cascade Amplification
In this study,the DNAzyme assisted signal amplification strategy was developed for simple,rapid and highly sensitive detection of APE1.The experiment conditions inclu-ding reaction time and temperature were optimized.This method exhibits feasibility,excel-lent specificity and high sensitivity with a detection limit of 3.0×10-4 U·mL-1.