Preparation and Antitumor Activity of Nanoparticles Loaded with Fennel Volatile Oil
The preparation and antitumor activity of nanoparticles loaded with Fennel volatile oil were investigated in this study.Using Eudragit S100(ES100)as the carrier material,nanoparticles loaded with Fennel volatile oil were prepared by nano-precipitation method and the preparation technology was optimized by single factor experiment.The morphology of nanoparticles was observed by transmission electron microscope and Malvern partilce size meter.The release in vitro was measured by dialysis method.The stability of nanoparticles at different temperatures was investigated.The effect of nanoparticles loade with Fennel volatile oil on proliferation,migration and invasion of human colon cancer RKO cells were detected by MTT assay,scratch assay and Transwell assay,respectively.The results showed that the optimal preparation conditions were as follows:The volume ratio of organic phase to aqueous phase was 1∶2,the concentration of ES100 was 14 mg·mL-1,the stir-ring speed was 1 500 r·min-1,and the dosage ration of Fennel volatile oil to ES100 was 4∶7.The prepared nanoparticles loaded with Fennel volatile oil had good morphology,high encapsulation rate and drug loading,and can be released in the enviroment of pH7.4 that was suitable to play a role in the colon.The nanoparticles were stable for 5 weeks at 25℃.It was also found that nanoparticles loaded with Fennel volatile oil could significantly inhibit the proliferation,migration and invasion of colon cancer RKO cells.This study provides an important reference for the preparation development and pharmacological application of fen-nel volatile oil.
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