In order to analyze the biodiversity and enzyme activity of cultivable bacteria in soils of Changyi National Marine Ecological Special Reserve.93 strains were isolated by 10-fold serial dilution method,and identified by 16S rRNA genes.Enzyme activities(including amylase,protease and cellulase)and NaCl tolerance were detected.Phylogenetic analysis based on 16S rRNA showed that the 93 bacteria belonged to 3 classes and 7 genera.The pre-dominant genera were Bacillus,comprising 50%of all bacteria.The results of enzyme ac-tivity test shows that the number of strains with cellulase activity,protease activity and am-ylase activity are 60,51 and 50,respectively.What is more,34 strains of them had 3 enzyme activi-ties.This study showed that the biodiversity of cultivable bacteria in soil of Changyi wetland,and some salt-tolerant strains with enzyme activities were obtained.Those provided a basis refer-ence for the study of environmental governance and purification capacity in coastal wetland.
维普
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