Microbial mixed culture for efficient degradation of polycyclic aromatic hydrocarbons using analytical hierarchy process
Five strains of efficient polycyclic aromatic hydrocarbons(PAHs)degrading bacte-ria were isolated from intertidal zone sediments,and the efficient PAHs degrading bacteria group was constructed based on the analytical hierarchy process(AHP).The AHP was used to compare the degradation rate and metabolic activity of bacteria in pairs,and three groups of PAHs degrading bacteria were constructed through different evaluation methods,namely AHP-1(subjective mild evaluation group),AHP-2(subjective extreme evaluation group)and AHP-3(objective evaluation group).The experimental results indicate that the degrada-tion rate of the microbial community is significantly higher than that of a single bacterium.On this basis,a control group was constructed by mixing bacteria in an equal proportion,and the degradation performance and metabolic characteristics of the above four bacterial groups were studied.The results showed that within one day,AHP-3 achieved a high degra-dation rate of PAHs(64.02%),which was higher than that of AHP-1(58.75%),AHP-2(55.17%)and the control group(54.98%).The AHP,a new method of constructing flora that has not yet been reported,can improve the degradation rate of PAHs.Therefore,it can be used as a model method to provide a theoretical basis for the construction of flora.
polycyclic aromatic hydrocarbons(PAHs)Biolog-ECO plate analysisanalytic hierarchy process(AHP)construction of strain