Objective To investigate the effect of pure titanium surface-loaded amorphous calcium polyphosphate(Ca-polyP)coating on osteogenic differentiation.Methods TA2 titanium sheets were taken,coated with Ca-polyP on the surface,and characterized by field emission scanning electron microscopy,X-ray energy dispersive spectroscopy,X-ray diffractometry,Fou-rier transform infrared spectroscopy,and contact angle measurement.Mouse embryo osteoblast precursor cells were inoculated on pure titanium sheets(control group)and titanium sheets loaded with Ca-polyP coating(experimental group)for cell culture.The proliferation activity of cells was measured by CCK-8 assay.The osteogenic differentiation ability of cells on the surface of titanium sheets in both groups was assessed by alkaline phosphatase activity assay and alkaline phosphatase staining.Results Successful loading of 20-chain-length Ca-polyP coating on titanium sheet surface was demonstrated by various physical characterization me-thods.The results of CCK-8 assay showed that titanium sheets in both groups were non-cytotoxic,and the cell proliferation activity on the surface of titanium sheets in the experimental group was significantly higher than that in the control group after 7 days of co-culture(F=1 375.183,P＜0.001).With the extension of co-culture time,the alkaline phosphatase activity of cells on the surface of titanium sheets gradually increased in both groups,and the alkaline phosphatase activity of cells on the surface of titanium sheets in the experimental group was significantly higher than that in the control group after 7 days of co-culture(F=41.141,P＜0.01).Al-kaline phosphatase staining followed by inverted fluorescence mi-croscopy showed that there were significantly more blue-purple dark-ly stained nodules on titanium sheets in the experimental group than in the control group.Conclusion Titanium sheets loaded with Ca-polyP coating on the surface significantly enhances the proliferation and osteogenic differentiation of mouse embryo osteoblast precursor cells compared with pure titanium sheets.
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