Objective To investigate the expression profile of plasma exosomal miRNAs and their application value in pa-tients with cerebral small vascular disease(CSVD).Methods Blood samples were collected from 66 patients with CVSD and 66 healthy controls,and then plasma exosomes were isolated.Plasma exosomes were randomly selected from 16 patients with CVSD and 16 healthy controls,and gene microarray was used to construct a library of differentially expressed miRNAs.PCR was per-formed for all 132 samples to verify the results of sequencing.Gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes analysis were performed.Results Gene microarray showed a total of 38 differentially expressed miRNAs in plas-ma exosomes of CVSD patients,among which 18 were upregulated and 20 were downregulated.Compared with the healthy control group,the patients with CVSD had significantly downregulated miR-498,miR320e,miR-6776,and miR-455.The results of quan-titative real-time PCR were consistent with gene microarray results.The patients with CVSD had a significantly downregulated ex-pression level of plasma exosomal miR-320e compared with the healthy control group(t=4.661,P＜0.001).The bioinformatics analysis showed that the target genes of differentially expressed miRNAs were involved in the biological processes including cell apoptosis,Alzheimer's disease,the FoxO signaling pathway,and the Wnt signaling pathway.Conclusion There is a significant difference in the expression profile of plasma exosomal miRNAs between CVSD patients and healthy controls,and miRNAs may be involved in the development and progression of CVSD through the regulation of target genes and biological signaling pathways.
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