载ADSCs的GelMA水凝胶对糖尿病大鼠伤口愈合的作用
Effect of ADSCs-loaded GelMA hydrogel on wound healing in diabetic rats
王玥琪 1赵志远 2高菲 1项锋钢 1迟菁华1
作者信息
- 1. 青岛大学基础医学院病理学系,山东青岛 266071
- 2. 青岛大学附属医院介入放射科,山东青岛 266071
- 折叠
摘要
目的 探究载脂肪间充质干细胞(ADSCs)的甲基丙烯酸酰化明胶(GelMA)水凝胶支架对糖尿病大鼠皮肤创伤愈合的影响.方法 将ADSCs接种在GelMA支架表面.用链脲佐菌素构建糖尿病大鼠模型,在大鼠背部建立直径为2 cm的圆形全层皮肤缺损,将大鼠分为对照组(未行治疗)、支架组(单独应用支架治疗)及支架细胞组(应用载ADSCs支架治疗).观察各组大鼠背部伤口愈合情况,采用苏木精-伊红染色对伤口皮肤组织进行组织学观察,采用免疫组织化学染色检测转化生长因子-β1(TGF-β1)表达.结果 与对照组及支架组相比,支架细胞组在术后第14、21天创面愈合率更高,在术后第14天TGF-β1表达更强,在术后第21天毛细血管破裂出血减少.结论 载ADSCs的GelMA水凝胶支架具有促进糖尿病大鼠伤口愈合的作用,TGF-β1可能在其中起重要作用.
Abstract
Objective To investigate the effect of adipose-derived stem cells(ADSCs)-loaded Gelatin Methacrylate(GelMA)hydrogel scaffolds on skin wound healing in diabetic rats.Methods ADSCs were seeded on the surface of GelMA scaf-folds.Streptozocin was used to establish a rat model of diabetes,and a round full-thickness skin defect with a diameter of 2 cm was created on the back of the rats.The rats were divided into control group(without any treatment),GelMA group(treated with scaf-folds alone),and ADSCs-loaded GelMA group(treated with ADSCs-loaded scaffolds).Wound healing was observed for each group;HE staining was used to observe the histology of skin tissue;immunohistochemical staining was used to measure the ex-pression of transforming growth factor-beta 1(TGF-β1).Results Compared with the control group and the GelMA group,the ADSCs-loaded GelMA group had a higher wound healing rate at 14 and 21 days after surgery,a stronger expression of TGF-β1 at 14 days after surgery,and a reduction in capillary bleeding at 21 days after surgery.Conclusion ADSCs-loaded GelMA hydrogel scaffolds can promote wound healing in diabetic rats,and TGF-β1 may play an important role in this process.
关键词
伤口愈合/糖尿病并发症/间质干细胞/水凝胶类/组织支架/病理学/大鼠,Sprague-DawleyKey words
wound healing/diabetes complications/mesenchymal stem cells/hydrogels/tissue scaffolds/pathology/rats,Sprague-Dawley引用本文复制引用
基金项目
教育部留学回国人员科研启动基金(80114020109)
出版年
2024
国家科技期刊平台
NSTL
万方数据