Objective To investigate the effect of stereotactic injection of the ferroptosis inducer RSL3 or Erastin in the ex-ternal segment of the globus pallidus(GPe)on the motor behavior of mice.Methods Male C57BL/6 mice,aged 8 weeks,were randomly divided into control group with 8 mice and experimental group with 24 mice(12 mice in Erastin group and 12 mice in RSL3 group).By means of the stereotactic injection technique,the mice in the experimental group were injected with 200 nL RSL3 or Erastin(with a concentration of 10 μmol/L)into the bilateral GPe,and thosein the control group were injected with an equal volume of normal saline.After two weeks,gait analysis was used to observethe motor behavior of mice,and Western blot was used to measure the protein expression levels of long-chain acyl-CoA synthetase 4(ACSL4)and glutathione peroxidase 4(GPX4)in mouse GPe.Results Compared with the control group,the Erastin group and the RSL3 group had a significant increase in the swing speed of mice,and the RSL3 group had significant changes in maximum contact area,footprint area,and stride length(F=5.433-7.486,q=4.039-5.446,P＜0.05).Compared with the control group,the Erastin group and the RSL3 group had a signifi-cant reduction in the protein expression level of GPX4 in the GPe of mice,and the RSL3 group had a significant increase in the pro-tein expression level of ACSL4(F=6.207,19.740;q=3.847-7.735;P＜0.01).Conclusion Stereotactic injection of the ferrop-tosis inducer RSL3 or Erastin in the GPe of mice can induce ferroptosis after two weeks,with enhanced motor behavior of mice.
维普
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