Objective To investigate the effects of intranasal administration of ferric ammonium citrate(FAC)on the olfac-tory function and microglia and astrocytes in the olfactory bulb(OB)of mice.Methods Twenty healthy 8-week-old male C57BL/6 mice were randomly divided into control group and FAC group,with tenmice in each group.The FAC group was treated with FAC solution through the nostrils alternately at a dose of 200 mg/kg,while the control group was given an equal volume of normal saline,once every other day for two weeks.Then,the olfactory function of the mice was tested,and the effects of in-tranasal administration of iron on microglia and astrocytes in the OB were examined with immunofluorescence staining.Results Compared with the control group,the FAC group showed significant reductionsin olfactory function(t=9.57,P＜0.05)and OB weight(t=10.50,P＜0.05)and significant increases in activated microglia(t=5.07,P＜0.05)and astrocytes(t=4.83,P＜0.05)in the OB.Conclusion Intranasal administration of FAC can cause olfactory dysfunction,impair the OB,and promote the acti-vation of microglia and astrocytes in the OB.
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