Objective To investigate the expression of Bassoon(BSN),a presynaptic cytomatrix protein,in glioma tissue and its influence on the prognosis of patients.Methods TCGA,CGGA,GEO,and CPTAC databases were used to download transcriptomic data,proteomic data,and the data of clinical samples,and the expression level of BSN in glioma tissue and its asso-ciation with prognosis were analyzed.The U251 cell line was transfected with siRNA to construct a glioma cell line with downregu-lated BSN expression.Western blotting was used to measure the influence of BSN on the expression levels of the proliferation-re-lated protein PCNA and the migration-related protein CD44 in glioma cells.Results The mRNA expression level of BSN was downregulated in glioma tissue compared with normal brain tissue(P＜0.001),and the abnormally low expression of BSN was significantly associated with the poor prognosis of glioma patients(P＜0.001).The mRNA expression level of BSN gradually de-creased with the increase in the grade of glioma.In addition,the protein expression level of BSN in high-grade glioma was signifi-cantly lower than that in the normal group(P＜0.001).Western blotting showed that knockdown of BSN significantly upregulated the protein expression levels of PCNA and CD44 in U251 cells(F=14.56,164.90;P＜0.01).Conclusion BSN expression is downregulated in glioma and is associated with tumor prognosis.Knockdown of BSN can promote the expression of proliferation and migration-related proteins in glioma cells,and BSN may play the role of a tumor suppressor gene in the development and pro-gression of glioma.
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