Objective To investigate the changes inastrocytes,microglial cells,and iron deposition in the substantia nigra(SN)and the externalglobus pallidus(GPe)in a mouse model of Parkinson's disease(PD)induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Healthy male C57BL/6 mice,aged 8 weeks,were randomly divided into control group and MPTP group.The mice in the MPTP group were intraperitoneally injected with MPTP at a dose of 18 mg/kg body weight,while those in the control group were given an equal volume of normal saline,twice a week for 5 weeks.The pole test was used to measure the motor function of mice;immunofluorescent staining was used to measure the numbers of tyrosine hydroxylase(TH)neurons,astrocytes,and microglial cells;Prussian blue iron staining was used to observe iron deposition.Results Compared with the control group,the MPTP group had a significant increase in the time to turn around(t=2.42,P＜0.05),a significant re-duction in the number of TH neurons and significant increases in the numbers of astrocytes,microglial cells,and iron-positive cells in the SN(t=3.82-4.83,P＜0.05),and a significant increase in the number of microglial cells in the GPe(t=2.54,P＜0.05),but there were no significant changes in the numbers of astrocytes and iron-positive cells in the GPe.Conclusion The mouse model of PD induced by MPTP is successfully established.There are increases in glial cell activation and iron deposition in the SN of model mice,while there is only an increase in microglial cell activation in the GPe.
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