MPTP慢性帕金森病模型小鼠步态的改变

Gait changes in a mouse model of chronic Parkinson's disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine

修民霞 ¹谢如意 ¹谢俊霞 ¹石丽敏¹

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作者信息

1. 青岛大学基础医学院生理学与病理生理学系,山东青岛 266071
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摘要

目的探究1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)慢性帕金森病(PD)模型小鼠步态改变.方法模型组8周龄雄性C57BL/6小鼠给予腹腔注射MPTP(30 mg/kg体质量,每周2次,共4周),对照组注射等量的生理盐水,每组10只.采用动物步态分析系统(CatWalk)分别检测两组注射后1、2、3和4周小鼠步态相关指标.结果步态分析结果显示,与对照组小鼠相比,模型组的运动持续时间、站立时间、脚步周期显著增加,平均速度、身体速度显著降低,差异均有统计学意义(t=2.209～5.176,P＜0.05).提示小鼠的步态发生改变.结论 MPTP慢性模型可以引起小鼠步态变化.

Abstract

Objective To investigate gait changes in a mouse model of chronic Parkinson's disease(PD)induced by 1-met-hyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Male C57BL/6 mice,aged 8 weeks,were intraperitoneally injec-ted with MPTP(30 mg/kg)twice a week for 4 weeks,and the mice in the control group were injected with an equal volumeof nor-mal saline,with 10 mice in each group.The animal gait analysis system(CatWalk)was used to measure gait-related parameters at 1,2,3,and 4 weeks after injection.Results The results of gait analysis showed that compared with the control group,the model group had significant increases in run duration,stance,and step cycle and significant reductions in average speed and body speed(t=2.209-5.176,P＜0.05).These results suggested gait changes in mice.Conclusion Gait changes are observed in a mouse model of chronic PD induced by MPTP.

关键词

帕金森病/1-甲基-4-苯基-1,2,3,6-四氢吡啶/步态分析/模型,动物/小鼠,近交C57BL

Key words

Parkinson disease/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/gait analysis/models,animal/mice,in-bred C57BL

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基金项目

国家自然科学基金面上项目(32171132)

出版年

2024

青岛大学学报(医学版)

青岛大学医学院

青岛大学学报(医学版)

CSTPCD

影响因子：0.8

ISSN：1672-4488

参考文献量1

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