Objective To investigate the biocompatibility of chitosan-based thermosensitive hydrogel carrier(4D-CTH)with adipose-derived mesenchymal stem cells(ADSCs)and its repair effect on full-layer skin wounds.Methods The enzyme digestion method was used to extract primary rabbit ADSCs,and flow cytometry and multidirectional differentiation were used for identifica-tion.4D-CTH and ADSCs were co-cultured in vitro,and live/death cell staining,CCK-8 assay,and fluorescent scratch assay were used to assess the biocompatibility of 4D-CTH.A model of full-layer skin defect was established,and rabbits were randomly divi-ded into Control group(wound was treated with 100 μL PBS buffer),ADSCs group(wound was treated with 100 μL ADSCs sus-pension),and 4D-CTH+ADSCs group(wound was treated with 4D-CTH-reproduced ADSCs).After 14 d of treatment,photos were taken to observe the wound healing rate of each group.Results The rabbit ADSCs sextracted were spindle-shaped,with positive expression of the surface marker molecules CD44 and CD90 and negative expression of the endothelial cell marker CD45 and the macrophage marker CD11b,and ADSCs had the potential of multidirectional differentiation.Live/dead cell staining showed that group,time,and the interaction effect of group and time had no effect on cell viability(Fgroup=0.716,P＞0.05;Ftime=1.219,P＞0.05;Fgroupxtime=1.567,P＞0.05).CCK-8 assay showed that group and the interaction effect of group and time had no effect on cell proliferation rate(Fgroup=1.995,P＞0.05;Fgroup×time=0.321,P＞0.05),and there was a significant difference in cell proliferation rate between different time points(Ftime=1 408.000,P＜0.01).Fluorescent scratch assayshowed that group and the interaction effect of group and time had no effect on cell migration rate(Fgroup=2.184,P＞0.05;Fgroup×time=0.108,P＞0.05),and there was a significant difference in cell migration rate between different time points(Ftime=50.110,P＜0.01).Wound healing assay showed that the 4D-CTH+ADSCs group had the highest wound healing rate(F=9.778-49.910,P＜0.01).Conclusion 4D-CTH-re-produced ADSCs can accelerate skin wound healing and promote skin regeneration.
维普
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