Objective To investigate the anti-inflammatory effect of Licochalcone A(LA)on Aspergillus fumigatus(AF)keratitis in mice.Methods CCK-8 assay was used to observe the effect of different concentrations of LA solution(12,24,36,48,and 60 μmol/L)on the proliferative activity of murine macrophages(RAW264.7);immunofluorescence assay was used to measure the degree of neutrophil infiltration in the cornea of mice on day 3 of AF infection;quantitative real-time PCR and enzyme-linked immunosorbent assay were used to measure the mRNA and protein expression levels of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-a(TNF-a),and heme oxygenase(HO-1)in RAW264.7 cells.Results LA at the concentration of 60 μmol/L had no toxicity to RAW264.7 cells(P＞0.05)and significantly reduced neutrophil infiltration in the cornea after in-fection(t=5.94,P＜0.01).LA at the concentration of 60 μmol/L significantly downregulated the mRNA and protein expression levels of IL-6,IL-1β,and TNF-α induced by AF(F=15.35-507.53,P＜0.001)and upregulated the mRNA expression of HO-1(F=1 633.06,P＜0.001).Conclusion LA exerts an anti-inflammatory effect in AF infection by reducing neutrophil infiltra-tion,downregulating the expression of inflammatory factors,and enhancing the expression of HO-1.
维普
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