D-AP5对PD小鼠黑质多巴胺能神经元簇状放电影响
Effect of intracerebroventricular injection of D-AP5 on burst firing of dopaminergic neurons in the substantia nigra in a mouse model of Parkinson's disease
赵继虎 1刘恒 2孙鹏1
作者信息
- 1. 青岛大学附属医院神经外科,山东青岛 266555
- 2. 青岛大学护理学院
- 折叠
摘要
目的 探究侧脑室注射D(-)-2-氨基-5-磷戊酸(D-AP5)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)帕金森病(PD)模型小鼠黑质区多巴胺能神经元簇状放电的影响.方法 将小鼠随机分为对照组、D-AP5组、MPTP组和D-AP5+MPTP组.采用电生理实验记录小鼠黑质区多巴胺能神经元的簇状放电百分比.结果 析因设计方差分析显示,D-AP5和MPTP两者存在交互作用(FMPTP=4.601,P＜0.05;FD-AP5=2.399,P＞0.05;F MPTP×D-AP5=12.020,P＜0.01).单独效应分析显示,在未注射MPTP的两组小鼠中,D-AP5组小鼠黑质区多巴胺能神经元簇状放电百分比与对照组小鼠相比差异无显著性(F=1.916,P＞0.05);在注射MPTP的两组小鼠中,D-AP5+MPTP组小鼠黑质区多巴胺能神经元簇状放电百分比较MPTP组明显降低(F=12.094,P＜0.01).结论 侧脑室注射D-AP5可降低MPTP模型小鼠黑质区多巴胺能神经元簇状放电百分比.
Abstract
Objective To investigate the effect of intracerebroventricular injection of D(-)-2-amino-5-phosphonopentanoic acid(D-AP5)on the burst firing of dopaminergic neurons in the substantia nigra ina mouse model of Parkinson's disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Mice were randomly divided into control group,D-AP5 group,MPTP group,and D-AP5+MPTP group.The bursting rate of substantia nigra dopaminergic neurons was recorded through the electrophysiological test.Results The factorial analysis of variance indicated an interaction between D-AP5 and MPTP(FMPTP=4.601,P＜0.05;FD-AP5=2.399,P＞0.05;FMPTP×D-AP5=12.020,P＜0.01).The individual effect analysis showed that there was no significant difference in the bursting rate of substantia nigradopaminergic neurons between the D-AP5 group and the control mice(F=1.916,P＞0.05);and the D-AP5+MPTP group showed a significantly lower bursting rate compared with the MPTP group(F=12.094,P＜0.01).Conclusion Intracerebroventricular injection of D-AP5 can reduce the bursting rate of do-paminergic neurons in the substantia nigra of MPTP model mice.
关键词
帕金森病/受体,N-甲基-D-天冬氨酸/兴奋性氨基酸拮抗剂/黑质/多巴胺能神经元/电生理学/小鼠,近交C57BLKey words
Parkinson disease/receptors,N-methyl-D-aspartate/excitatory amino acid antagonists/substantia nigra/do-paminergic neurons/electrophysiology/mice,inbred C57BL引用本文复制引用
基金项目
国家自然科学基金青年科学基金项目(32000795)
中国博士后基金面上项目(2017M622129)
山东省自然科学基金青年科学基金项目(ZR2022MH022)
山东省自然科学基金青年科学基金项目(ZR2020QC095)
出版年
2024
国家科技期刊平台
NSTL
万方数据