Exendin-4对小鼠黑质网状部神经元自发放电影响
Effect of Exendin-4 on the spontaneous discharge of neurons in the substantia nigra pars reticulata of mice
沈方帅 1李筱雪 1刘翠 1陈心怡 2薛雁 1陈蕾1
作者信息
- 1. 青岛大学医学部基础医学院生理学与病理生理学系,山东青岛 266071
- 2. 青岛大学附属医院国际医疗中心,山东青岛 266071
- 折叠
摘要
目的 探究胰高血糖素样肽-1(GLP-1)受体激动剂Exendin-4对小鼠黑质网状部神经元自发放电的影响.方法 采用免疫荧光染色观察GLP-1受体(GLP-1R)在小鼠黑质网状部的表达.使用在体电生理微电极注射10 μmol/L的Exendin-4到小鼠黑质网状部神经元表面,观察黑质网状部非多巴胺能(假定为γ-氨基丁酸能)神经元的自发放电变化.结果 免疫荧光染色结果显示,小鼠黑质网状部有GLP-1R的高表达.在所记录到的13个黑质网状部神经元中,Exendin-4可显著降低其中9个神经元的自发放电,其放电频率由加药前(18.62±3.19)Hz降低到(14.615±3.06)Hz,差异有显著性(t=7.257,P＜0.05);其放电频率平均降低(21.85±7.72)％,较生理盐水组的(1.15±9.67)％显著升高(Z=2.282,P＜0.05).在另外的4个黑质网状部神经元中,Exendin-4对放电频率无明显影响,其放电频率由(13.49±7.16)Hz变为(13.54±7.45)Hz,差异无显著性.结论 Exendin-4能降低部分黑质网状部神经元的自发放电频率.
Abstract
Objective To investigate the effectof Exendin-4,a glucagon-like peptide-1(GLP-1)receptor agonist,on the spontaneous discharge of neurons in the substantia nigra pars reticulate of mice.Methods Immunofluorescent staining was used to observe the expression of GLP-1receptor(GLP-1R)in the substantia nigra pars reticulata of mice.Extendin-4 10 μmol/L were injected onto the surface of neurons in the substantia nigra pars reticulate using in vivo electrophysiological microelectrodes to ob-serve the spontaneous discharge of non-dopaminergic neurons(assumed to be GABAergic neurons)in the substantia nigra pars re-ticulate.Results Immunofluorescent staining showed a high expression level of GLP-1R in the substantia nigra par reticulate of mice.As for the 13 neurons recorded in the substantia nigra par reticulata,Exendin-4 significantly reduced the spontaneous dis-charge rate of 9 neurons,with a significant reduction in discharge frequency from(18.62±3.19)Hz to(14.615±3.06)Hz(t=7.257,P＜0.05),and the discharge frequency was reduced by(21.85±7.72)％on average,which was significantly higher than the reduction rate of(1.15±9.67)％in the normal saline group(Z=2.282,P＜0.05).In the other 4 neurons,Exendin-4 had no signi-ficant effect on discharge frequency,which changed from(13.49±7.16)Hz to(13.54±7.45)Hz.Conclusion Exendin-4 can re-duce the spontaneous discharge frequency of some neurons in the substantia nigra pars reticulata.
关键词
艾塞那肽/黑质/网状部/胰高血糖素样肽-1受体/电生理学/小鼠,近交C57BLKey words
exenatide/substantia nigra/pars reticulata/glucagon-like peptide-1 receptor/electrophysiology/mice,inbred C57BL引用本文复制引用
基金项目
国家自然科学基金青年科学基金项目(32200939)
山东省自然科学基金资助项目(ZR2022QC225)
山东省自然科学基金资助项目(ZR2023MC072)
泰山学者青年专家计划资助项目(tsqnz20230634)
出版年
2024
国家科技期刊平台
NSTL
万方数据