UCH-L1和GFAP对硬膜外血肿进展的预测价值
Value of ubiquitin C-terminal hydrolase-L1 and glial fibrillary acidic protein in predicting the progression of epidural hematoma
刘俊汝 1张广宇 1邓明铷 2聂安格 1袁海成3
作者信息
- 1. 潍坊医学院临床医学院,山东潍坊 261000
- 2. 青岛大学医学部
- 3. 康复大学青岛中心医院(青岛市中心医疗集团)神经二科
- 折叠
摘要
目的 探讨泛素C末端水解酶-L1(UCH-L1)和胶质纤维酸性蛋白(GFAP)在预测硬膜外血肿(EDH)进展中的价值.方法 将雄性新西兰白兔随机分为假手术组(SH组)和EDH 0.1、0.2、0.3 mL组,每组各24只.于术后1、3、6、24 h采血,采用酶联免疫吸附试验法测定血浆中UCH-L1、GFAP的水平.术后24 h进行神经行为学评分、脑含水量检测,采用免疫组织化学法检测大脑皮质中UCH-L1及GFAP的表达.结果 与SH组相比,EDH各组的神经行为学评分降低(F=290.10,P＜0.001)、脑含水量增加(F=33.64,P＜0.001).4组兔大脑皮质中UCH-L1和GFAP的差异有统计学意义(F=45.90、118.03,P＜0.001).EDH各组血浆UCH-L1水平在术后1、3、6 h高于SH组(F=3.15～47.93,P＜0.001),血浆GFAP水平在术后各个时间点均高于SH组(F=29.17～332.00,P＜0.001).结论 EDH体积增大可以促进损伤侧大脑皮质中UCH-L1和GFAP的表达,上调血浆中二者的水平.大脑皮质和血浆中的UCH-L1和GFAP可作为预测EDH进展的有效生物标志物.
Abstract
Objective To investigate the value of ubiquitin C-terminal hydrolase-L1(UCH-L1)and glial fibrillary acidic pro-tein(GFAP)in predicting the progression of epidural hematoma(EDH).Methods Male New Zealand white rabbits were ran-domly divided into sham-operation group(SH group),EDH 0.1 mL group,EDH 0.2 mL group,and EDH 0.3 mL group.Blood samples were collected at 1,3,6,and 24 h after surgery,and enzyme-linked immunosorbent assay was used to measure the levels of UCH-L1 and GFAP in plasma.Neurobehavioral scoring and brain water content measurement were performed at 24 h after sur-gery,and immunohistochemistry was used to measure the expression of UCH-L1 and GFAP in cerebral cortex.Results Compared with the SH group,the three EDH groups had a significant reduction in neurobehavioral score(F=290.10,P＜0.001)and a significant increase in brain water content(F=33.64,P＜0.001).There were significant differences in UCH-L1 and GFAP in cerebral cortex between the four groups(F=45.90,118.03;P＜0.001).Compared with the SH group,the three EDH groups had a significantly higher level of UCH-L1 in plasma at 1,3,and 6 h after surgery(F=3.15-47.93,P＜0.001)and a significantly higher level of GFAP in plasma at each time point after surgery(F=29.17-332.00,P＜0.001).Conclusion An increase in EDH volume can promote the expression of UCH-L1 and GFAP in the injured cortex and upregulate the levels of UCH-L1 and GFAP in plasma.UCH-L1 and GFAP in cerebral cortex and plasma can be used as effective biomarkers for predicting the progres-sion of EDH.
关键词
血肿,硬膜外,颅内/生物标记/泛素C末端水解酶-L1/神经胶质原纤维酸性蛋白质Key words
hematoma,epidural,cranial/biomarkers/ubiquitin C-terminal hydrolase-L1/glial fibrillary acidic protein引用本文复制引用
基金项目
国家自然科学基金资助项目(81673360)
出版年
2024
国家科技期刊平台
NSTL
万方数据