高尿酸血症小鼠模型构建及其对肠屏障损伤的影响
Establishment of a mouse model of hyperuricemia with intestinal barrier injury
朱现菊 1吕秋兰 2胡鹤鸣1
作者信息
- 1. 青岛市第八人民医院检验科,山东青岛 266000
- 2. 青岛大学附属医院医学研究中心
- 折叠
摘要
目的 构建稳定的肠屏障损伤高尿酸血症小鼠模型.方法 采用不同浓度腺嘌呤(Ade)和氧嗪酸钾(PO)灌胃小鼠,分别在第3、7、14和21天检测血清尿酸、尿素氮、肌酐水平和肠渗透性,检测空肠、回肠和结肠中紧密连接蛋白Occludin、ZO-1和Claudin-1的表达.结果 高浓度的Ade和PO导致小鼠尿酸升高(F=25.453～518.039,P＜0.01)、体质量降低(F=6.900～43.724,P＜0.05).50 mg/kg Ade 和 125 mg/kg PO 连续灌胃 7 d 导致小鼠尿酸升高(P＜0.05),可持续升高至21 d(P＜0.05),同时肠渗透性升高(F=28.563～185.808,P＜0.05)并与尿酸水平呈正相关性(r=0.876 9,P＜0.05).与正常小鼠相比,Occludin和ZO-1在高尿酸血症小鼠回肠和结肠中表达降低(t=3.164、3.678,P＜0.05),Claudin-1在空肠中表达降低(t=2.670,P＜0.05).结论 采用50 mg/kg Ade和125 mg/kg PO连续灌胃7 d可构建肠屏障损伤的高尿酸血症小鼠模型.
Abstract
Objective To establish a stable mouse model of intestinal barrier injury induced by hyperuricemia.Methods The mice were given different concentrations of adenine(Ade)and potassium oxazinate(PO)by gavage,and serum uric acid,urea nitrogen,creatinine,and intestinal permeability were measured on days 3,7,14,and 21.The expression levels of tight junction proteins Occludin,ZO-1,and Claudin-1 in the jejunum,ileum,and colon were measured.Results The high concentrations of Ade and PO induced a significant increase in uric acid(F=25.453-518.039,P＜0.01)and a significant reduction in body weight(F=6.900-43.724,P＜0.05).Administration of 50 mg/kg Ade and 125 mg/kg PO by gavage for 7 consecutive days induced a significant increase in uric acid(P＜0.05),which continued to rise until day 21(P＜0.05),and there was also a significant increase in intestinal permeability(F=28.563-185.808,P＜0.05),which was positively correlated with the level of uric acid(r=0.876 9,P＜0.05).Compared with normal mice,the mice with hyperuricemia had significant reductions in the expression levels of Occludin and ZO-1 in the ileum and the colon(t=3.164,3.678;P＜0.05),as well as a significant reduction in the expression level of Clau-din-1 in the jejunum(t=2.670,P＜0.05).Conclusion Administration of 50 mg/kg Ade and 125 mg/kg PO by gavage for 7 consecutive days can establish a mouse model of hyperuricemia with intestinal barrier injury.
关键词
高尿酸血症/肠/容积渗克分子浓度/疾病模型,动物Key words
hyperuricemia/intestines/osmolar concentration/disease models,animal引用本文复制引用
基金项目
国家自然科学基金资助项目(81901575)
出版年
2024
国家科技期刊平台
NSTL
万方数据