摘要
目的 探讨发生重症急性胰腺炎(SAP)时5-羟色胺(5-HT)在肠黏膜屏障损伤中的作用.方法 将大鼠随机分为假手术组(SO组)、模型组(SAP组)和干预组(SAP+对氯苯丙氨酸(PCPA)组),每组10只.通过胆胰管内注射50 g/L牛磺胆酸钠诱导SAP大鼠模型.SAP大鼠模型构建后24 h时检测各组大鼠血清脂肪酶、淀粉酶、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、5-HT水平以及回肠组织5-HT含量,采用苏木精-伊红染色评估胰腺和回肠的形态学变化,采用Western blot方法和免疫荧光技术检测回肠组织紧密连接蛋白(occludin、clau-din-1和ZO-1)的表达.结果 SAP组大鼠的血清脂肪酶、淀粉酶、TNF-α、IL-6水平以及回肠组织5-HT含量较SO组明显增高,SAP+PCPA组上述指标较SAP组明显下降(F=34.54～479.90,P＜0.01).各组胰腺和肠道损伤病理评分比较,SAP组明显高于SO组,SAP+PCPA组显著低于SAP组(F=207.80、33.69,P＜0.01).SAP组回肠组织中occludin、claudin-1和ZO-1蛋白的表达较SO组显著减少,SAP+PCPA组上述蛋白的表达较SAP组显著升高,差异具有统计学意义(F=10.29～23.63,P＜0.01).结论 SAP发生时抑制肠道5-HT的产生可以减轻肠黏膜屏障损伤.
Abstract
Objective To investigate the role of 5-hydroxytryptamine(5-HT)in intestinal mucosal barrier injury in severe acute pancreatitis(SAP).Methods Rats were randomly divided into sham-operation group(SO group),model group(SAP group),intervention group(SAP+p-chlorophenylalanine(PCPA)group),with 10 rats in each group.A rat model of SAP was in-duced by injecting 50 g/L sodium taurocholate into the biliopancreatic duct.At 24 h after the construction of the rat model of SAP,each group of rats was tested for the levels of serum lipase,amylase,tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),and 5-HT and the content of 5-HT in the ileal tissue.Morphological changes inthe pancreas and small intestine were evaluated by hematoxylin-eosin staining.Western blot and immunofluorescence techniques were used to determine the expression of tight junc-tion proteins(occludin,claudin-1,and ZO-1)in the ileal tissue.Results Compared with the SO group,the SAP group had sig-nificantly increased levels of serum lipase,amylase,TNF-α,and IL-6 and content of 5-HT in the ileal tissue.Compared with the SAP group,the SAP+PCPA group had significant reductions in the above indices(F=34.54-479.90,P＜0.01).The pathological scores of pancreatic and intestinal injury were significantly higher in the SAP group than in the SO group and were significantly lo-wer in the SAP+PCPA group than in the SAP group(F=207.80,33.69,P＜0.01).Meanwhile,the protein expression of occlu-din,claudin-1,and ZO-1 was significantly lower in the SAP group than in the SO group and was significantly higher in the SAP+PCPA group than in the SAP group(F=10.29-23.63,P＜0.01).Conclusion The production of intestinal 5-HT is inhibited during the development of SAP,which can reduce the intestinal mucosal barrier injury.
维普
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