Objective To explore the effects of circPCMTD1 on the proliferation,migration,invasion,and apoptosis of acute myeloid leukemia(AML)cells and the underlying molecular mechanism.Methods Human bone marrow stromal cell line HS-5 and AML cell lines HL-60,THP-1,U-937,and Kasumi-1 were cultured in vitro.THP-1 cells were randomly divided into NC group,si-NC group,si-circPCMTD1 group,miR-328 NC group,miR-328-3p group,si-circPCMTD1+anti-miR-328 NC group,and si-circPCMTD1+anti-miR-328-3p group.Real-time fluorescence quantitative PCR and Western blot were used to mea-sure the expression of circPCMTD1,miR-328-3p,and related proteins.Cell proliferation,migration,invasion,and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),Transwell and flow cytometry methods.The relationship between circPCMTD1 and miR-328-3p was analyzed by dual luciferase reporting assay.Results Compared with HS-5 cells,circPCMTD1 was highly expressed in AML cells(F=66.258,P＜0.05),whereas the opposite was true for miR-328-3p(F=101.145,P＜0.05).Silencing circPCMTD1 inhibited the proliferation,migration,and invasion of cells and the expression of related proteins,and induced cell apoptosis(F=38.952-290.338,P＜0.05).Upregulation of miR-328-3p impaired the luciferase activity of WT-circPCMTD1,and downregulation of miR-328-3p attenuated the inhibitory effect of circPCMTD1 downregulation on cell proliferation(F=42.655,P＜0.05).Conclusion Silencing circPCMTD1 can repress THP-1 cell growth through targeting miR-328-3p.
维普
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