CXCL12通过CXCR4/AKT诱导自噬对肺癌细胞迁移的作用

CXCL12 induces autophagy and promotes the migration of lung cancer cells via the CXCR4/AKT signaling pathway

张一帆 ¹万里新 ²李慧 ³孙晓¹

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作者信息

1. 南阳市中心医院肺部肿瘤内科,河南南阳 473000
2. 南阳市中心医院肿瘤内科,河南南阳 473000
3. 南阳市中心医院科研科,河南南阳 473000
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摘要

目的探究外源性趋化因子配体12(CXCL12)通过激活趋化因子受体4(CXCR4)/蛋白激酶B(AKT)信号通路对肺癌细胞迁移的作用.方法用外源性CXCL12处理肺癌细胞,通过Western blot实验对自噬相关蛋白LC3B以及CXCR4/AKT信号通路蛋白进行检测,通过划痕实验对细胞的迁移进行检测.结果与对照组相比较,加入外源性CXCL12组细胞的迁移能力增强(t=3.949,P＜0.05),自噬相关蛋白LC3B Ⅱ的表达水平显著增高(t=3.051,P＜0.05),CXCR4的表达和AKT的磷酸化水平也明显增高(t=2.974、4.307,P＜0.05).结论 CXCL12可能通过CXCR4/AKT信号通路诱导自噬进而促进肺癌细胞的迁移.

Abstract

Objective To investigate the effect of exogenous CXCL12 on the migration of lung cancer cells by activating the CXCR4/AKT signaling pathway.Methods Lung cancer cells were treated by exogenous CXCL12.Western blot was used to measure the autophagy-related protein microtubule-associated protein light chain 3B Ⅱ(LC3B Ⅱ)and the proteins associated with the CXCR4/AKT signaling pathway,and wound healing assay was used to observe cell migration.Results Compared with the control group,the group with the addition of exogenous CXCL12 had significant increases in the migration ability of cells(t=3.949,P＜0.05)and the expression of the autophagy-related protein LC3B Ⅱ(t=3.051,P＜0.05),as well as significant increases in the expression of CXCR4 and the phosphorylation level of AKT(t=2.974,4.307,P＜0.05).Conclusion CXCL12 may pro-mote the migration of lung cancer cells by inducing autophagy via the CXCR4/AKT signaling pathway.

关键词

趋化因子CXCL12/受体,CXCR4/肺肿瘤/自噬/细胞运动

Key words

chemokine CXCL12/receptors,CXCR4/lung neoplasms/autophagy/cell movement

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出版年

2024

青岛大学学报(医学版)

青岛大学医学院

青岛大学学报(医学版)

CSTPCD

影响因子：0.8

ISSN：1672-4488

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