Objective To investigate the effect of repeated restraint stress on synaptic activity of pyramidal neurons in the medial prefrontal cortex(mPFC)of wild-type C57BL6/J mice.Methods Male C57BL6/J mice,aged 12-20 weeks,were randomly divided into control group and restraint group,and the mice in the restraint group were given repeated restraint stress,1 hour per day for 4 consecutive days.The elevated plus maze test was used to evaluate the anxiety-like behaviors of mice,and whole-cell patch clamp technique was used to record the excitatory and inhibitory postsynaptic currents of pyramidal neurons in the mPFC.Results The mice in the restraint group had a significant increase in anxiety-like behaviors(t=3.069,P＜0.01),as well as significant increases in the frequency of miniature excitatory postsynaptic currents(t=4.009,P＜0.01)and the amplitude of miniature inhibitory postsynaptic currentsin pyramidal neurons of the mPFC(t=2.172,P＜0.05).Conclusion Repeated re-straint stress can enhance the excitatory and inhibitory synaptic transmission in pyramidal neurons of the mPFC,and such plasticity changes in synaptic transmission of pyramidal neurons in the mPFC may be closely associated with the changes in anxiety-related behaviors caused by repeated restraint stress.
维普
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