Changes in intestinal flora and their association with the expression of monocyte chemoattractant protein-1 and growth regulating onco-gene-α in pregnant women with gestational diabetes meilitus complicated by premature rupture of membranes
Changes in intestinal flora and their association with the expression of monocyte chemoattractant protein-1 and growth regulating onco-gene-α in pregnant women with gestational diabetes meilitus complicated by premature rupture of membranes
Objective To investigate the changes in intestinal flora and their association with the expression of monocyte chemoattractant protein-1(MCP-1)and growth regulating oncogene-α(GRO-α)in peripheral blood in pregnant women with gesta-tional diabetes mellitus(GDM)complicated by premature rupture of membranes.Methods A total of 162 pregnant women with GDM complicated by premature rupture of membranes who attended Renmin Hospital,Hubei University of Medicine,from Ja-nuary 2020 to November 2023 were enrolled as observation group,100 pregnant women with simple GDM were enrolled as control group A,and 100 pregnant women with premature rupture of membranes were enrolled as control group B.The three groups were compared in terms of the distribution of intestinal flora and the expression of MCP-1 and GRO-α in peripheral blood,as well as their association with pregnancy outcome in the observation group.Results Compared with the control groups A and B,the ob-servation group had a significantly higher content of Enterococcus and significantly lower contents of Lactobacillus and Bifidobac-terium(F=12.212-30.595,P＜0.05).The observation group had significantly higher expression levels of MCP-1 and GRO-α in peripheral blood than the control groups A and B(F=11.543,15.922;P＜0.05).As for the observation group,compared with the pregnant women with non-adverse pregnancy outcomes,the pregnant women with adverse pregnancy outcomes had a significantly higher content of Enterococcus and significantly lower contents of Lactobacillus and Bifidobacterium(t=-8.804 to 8.736,P＜0.05),as well as significantly higher expression levels of MCP-1 and GRO-α in peripheral blood(t=7.278,11.133;P＜0.05).As for the observation group,compared with the pregnant women with a gestational age of 37 weeks,the pregnant women with a gestational age of＜37 weeks had a significantly higher content of Enterococcus and significantly lower contents of Lactobacillus and Bifidobacterium(t=-8.756 to 8.683,P＜0.05),as well as signifi-cantly higher expression levels of MCP-1 and GRO-α in peripheral blood(t=5.832,12.612;P＜0.05).The content of Enterococcus was positively correlated with the expression levels of MCP-1 and GRO-α(r=0.334,0.365;P＜0.05),and the contents of Lacto-bacillus and Bifidobacterium were negatively correlated with the expression levels of MCP-1 and GRO-α(r=-0.381 to-0.337,P＜0.05).Conclusion Pregnant women with GDM and premature rupture of membranes have intestinal flora disturbance and inflammatory response,which are more obvious in pregnant women with adverse pregnancy outcomes.The changes in intestinal flora are correlated with the expression levels of MCP-1 and GRO-α in peripheral blood.
维普
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