Effects of Xizang medicine Ershiwuwei Guijiu Pill on serum sex hor-mones and bone turnover markers in the ovariectomized rats
Objective To explore the effects of Xizang medicine Ershiwuwei Guijiu Pill on serum sex hor-mones and bone turnover markers in the ovariectomized rats.Methods 36 SD rats were randomly divided into med-icine group,model group and sham group.Ovariectomy was performed in the medicine group and model group.Two weeks after the surgery,the medication was administered by gavage,with the medicine group receiving a suspen-sion of Ershiwuwei Guijiu Pill(400 mg/kg),and the model group and sham operation group receiving distilled wa-ter once a day for 12 weeks.Micro-CT was used to detect the structure of the right proximal femur;SEM was used to observe the microstructure of the femur;ELISA was used to detect serum bone resorption marker tartrate-resis-tant acid phosphatase 5 b(TRAP5 b),serum bone formation marker serum bone-specific alkaline Phosphatase(BALP),serum estradiol(E2),luteinizing hormone(LH),follicle-stimulating hormone(FSH).Results The re-sults of Micro-CT and SEM showed that Ershiwuwei Guijiu Pill could significantly improve the proximal femur bone microstructure and bone trabecular density in the model of ovariectomized osteoporosis rats.The pearson cor-relation analysis resalts showed that there is a negative correlation between E2 levels and TRAP5 b levels,while se-rum E2 levels are positively correlated with BALP.There is a negative correlation between serum FSH,LH levels and BALP levels.Conclusion The Xizang medicine Ershiwuwei Guijiu Pill could improve the level of serum E2 in the ovariectomized rats and stimulate the feedback regulation of LH and FSH in the upstream of hypothalamic pitu-itary ovarian axis(HPO).In addition,it could also reduce the content of serum TRAP5 b and increase the content of serum BALP,which could improve bone metabolism imbalance and thereby improve bone microstructure.
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