Differences of intestinal microbes and its metabolites between individuals with altitude-related hypertension(ARH)and those with normal blood pressure
Objective To analyze the differences in intestinal microbes between individuals with altitude-re-lated hypertension(ARH)and those with normal blood pressure,and to explore the action mechanism of ARH.Methods 16S rDNA sequencing and metabolomics analysis were performed on the feces of ARH patients(n=7)and healthy controls(n=10).Spearman correlation analysis was adopted to explore the correlation between intesti-nal microbes and metabolomics.Results A total of 23 differential metabolites were detected from ARH group and the control group.Among them,9 metabolites such as 2,3-dihydroxy-3-methylbutyric acid,pantothenic acid,16-hydroxypalmitic acid and glycyl-l-leucine were downregulated;a total of 14 metabolites including uracil,2-oxa-dipic acid,DL-3-amino-isobutyric acid,histamine and daidzein were upregulated.A total of 11 differential meta-bolic pathways including primary bile acid biosynthesis were identified after KEGG metabolic pathway analysis.Through 16S rDNA sequencing analysis,three different bacteria were selected at the genus level,including Bifido-bacterium,Peptostreptococcus and Catenibacterium.The correlation analysis between differential bacteria and me-tabolites showed that both Streptococcus digesticus and Streptococcus were positively correlated with the level of lin-oleic acid.Bifidobacterium was negatively correlated with 20-hydroxy-arachidonic acid and glycine-L-leucine.Conclusion The elevated blood pressure in ARH group may be related to the downregulation of linoleic acid,pan-tothenic acid and 2,3-dihydroxy-3-methylbutyric acid caused by intestinal microbes imbalance.The upregulation of Bifidobacterium and other microorganisms,together with the body's enrichment of histamine,daidzein and other metabolites may alleviate the persistent increasing of blood pressure.
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