Obtaining biological function and signaling pathway information of Alveolar echinococcosis histone H3K4ME1 based on bioinformatics and animal experimental methods
Objective To obtain the biological function and signaling pathway information of Alveolar echi-nococcosis histone H3K4ME1 based on bioinformatics methods and animal experimental methods.Methods 1.Ex-tract the nuclei of frozen tissue.2.Build a cut&tag library.3.Analyze biological information:1)Evaluate the se-quencing data of alveolar echinococcosis histone H3K4ME1;2)Determine the distribution of reads on the genome;3)Analyze the enriched interval information of a single sample;4)Analyze peak transcription factors;5)Analyze the GO functional enrichment and KEGG pathway enrichment of genes associated with Peak;6)Analyze the basic situation of differentially associated genes;7)Analyze the GO functional enrichment of differentially associated genes;8)Analyze the enrichment of differentially associated genes in the KEGG pathway.Results 1.The extrac-tion of nuclei from frozen tissue:the nuclei count was>100 000 under the microscope,indicating successful ex-traction of the cell nuclei.2.After the sequencing library was constructed,the software qubit was used to check the sequencing library,and the results showed that it was qualified.3.Results of information analysis:1)The similarity between the experimental group and the reference genome was>98%(the sequencing data was good)for further testing;2)The reads of the alveolar echinococcosis group and the uninfected normal mice group showed signifi-cant peaks in TSS;3)Significant Peaks were identified through the enrichment screening of individual samples;4)Peak-associated transcription factors were mainly concentrated in zf-C2H2,Homeobox,and BHLH,etc.;5)The en-richment of the GO function of Peak-associated genes was mainly in the biological processes such as the localiza-tion of cellular proteins and macromolecules,cellular developmental regulation,and the regulation of anatomical structure and morphology.The KEGG pathway enrichment of Peak-associated genes was mainly enriched in MAPK,cGMP-PKG,Hippo and other signaling pathways;6)There were 5 547 differentially associated genes in histone H3K4ME1 between the alveolar echinococcosis group and the uninfected normal mice group,including 2 556 up-regulated genes and 2 929 down-regulated genes;7)The biological processes of GO functional enrich-ment analysis of histone H3K4ME1 differentially associated genes mainly included positive regulation of cellular projection tissue and GTPase activity regulation.The cellular components mainly included cell projection mem-brane,synaptic.The molecular functions mainly included actin binding,cell adhesion molecule binding.8)The KEGG pathway enrichment analysis of histone H3K4ME1 differentially associated genes was mainly distributed in the signaling pathways such as MAPK,Wnt and Rap1.Conclusion The information on the biological functions and signaling pathways of histone H3K4ME1 was obtained in the alveolar echinococcosis group.
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