Effects of Ectoine on HaCaT Cell Viability and AQP3,Claudin-1 and ZO-1 Expression
Objective The study investigates the effects of Ectoine on cell viability,apoptosis,and intracellu-lar reactive oxygen species(ROS)levels in human keratinocyte cells(HaCaT),as well as analyzing the expression levels of aquaporin 3(AQP3),claudin-1,and zonula occludens-1(ZO-1).Methods Experimental groups with varying concentrations of Ectoine and a blank control group were set up.HaCaT cells were cultured for 24 hours,and the cell viability was detected using the CCK8 method to determine the optimal concentration of Ectoine.The expression levels of AQP3,Claudin-1,ZO-1 in HaCaT cells were examined by Western Blot analysis,while the apoptosis rate and intracellular ROS levels in HaCaT cells were detected using flow cytometry.Results The CCK8 results showed that the cell viability in the 0.1%,0.2%,0.3%Ectoine groups were all higher than 120%,with the 0.2%Ectoine group demonstrating the highest level at 146.92%±7.67%.Compared with the blank control group,the experimental groups with different concentrations of Ectoine significantly increased the expression levels of AQP3,Claudin-1,and ZO-1 in HaCaT cells(P<0.05),and concurrently decreased both the apoptosis rate and in-tracellular ROS levels significantly(P<0.05).Conclusion Ectoine can enhance the cell viability of HaCaT cells,decrease their apoptosis rate and intracellular ROS levels,and upregulate the expression of AQP3,Claudin-1,and ZO-1.Ectoine may be related to the expression of relevant moisturizing proteins and exhibits a certain protective effect on HaCaT cells.
NETL
NSTL
万方数据
