Research on the protective effects and mechanism of Bawei Chenx-iang Powder on the cardiac injury induced by adriamycin based on network pharmacology and animal experiments
Objective To investigate the protective effects and mechanism of Bawei Chenxiang Powder on the cardiac injury induced by adriamycin based on network pharmacology and animal experiments.Methods The study was carried out based on network pharmacology and animal experiments.The blood-entry components and targets of Bawei Chenxiang Powder were screened by using TCMSP database.Disease targets were searched by us-ing databases such as OMIM and GeneCards.A PPI network was constructed by taking intersection targets.GO and KEGG enrichment analyses were performed,and the network of"blood-entry components-targets-associated path-ways-key diseases"was established.A model of adriamycin cardiac injury was established.The electrocardiogram and the left heart function of rats were detected.The levels of serum ALT,AST,LDH and CK-MB were measured.The expression of PI3K/AKT signaling pathway and apoptosis-related proteins in myocardial tissue were mea-sured.The ultrastructure of myocardium was observed.Results 1,897 blood-entry targets of Bawei Chenxiang Pow-der,1,598 targets of adriamycin cardiac injury and 198 intersection targets of Bawei Chenxiang Powder and adriam-ycin cardiac injury were screened,among which AKT1,STAT3,EGFR,SCR,BCL2,TNF,MAPK1,MAPK3,ESR1 and CASP3 were key targets.GO and KEGG analyses revealed that PI3K/AKT may be an important signaling pathway for Bawei Chenxiang Powder to improve the cardiac injury induced by adriamycin.The results showed that Bawei Chenxiang Powder could improve the left heart function of rats with adriamycin cardiac injury and reduce the lev-els of serum ALT,AST,LDH and CK-MB.In addition,it could upregulate PI3K,AKT,and BCL2 in rat myocardial tissue,downregulate BAX and CASP3 proteins and improve myocardial ultrastructure.Conclusion Bawei Chenx-iang Powder can effectively improve cardiac function in adriamycin heart-injured rats.Its possible action mecha-nism is to regulate PI3K/AKT signaling pathway so as to inhibit cell apoptosis.
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